News
June 12, 2013
Superresolution imaging is catching on in the popular science media! Check out this new article on superresolution microscopy in ScienceNews
March 24, 2013
Allele is excited to announce the release of our newest fluorescent protein, mNeonGreen, which is a super-performer in superresolution imaging!
mNeonGreen was developed in collaboration with our partner non-profit research institute, the Scintillon Institute.
Shown below to the right is a stochastic single-molecule superresolution (SSMS) image of mNeonGreen fused to the C terminus of human keratin. On the left is the same cell imaged with widefield TIRF microscopy. (Click on each image to see the full-sized image in a new window)
Publications
Allele Publications
1. Shaner, N.C. et al. "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature Methods 2013, ePub March 24. Download PDF
This paper describes mNeonGreen and shows several examples of its use in superresolution (SSMS) microscopy.
2. McEvoy A.L. et al. "mMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities." (2012) PLoS One 2012, 7(12):e51314. Download PDF
This paper describes our newest photoconvertible protein, mMaple, and gives examples of its use in different modes of superresolution imaging (STORM and STED).
3. Hoi H. et al. "A monomeric photoconvertible fluorescent protein for imaging dynamic protein localization." J Mol Biol 2010, Sep 3;401(5):776-91. Download PDF
This paper describes the development of mClavGR2, our first photoconvertible protein.
Other Useful References
A comprehensive list of highly useful references for every aspect of superresolution imaging and general microscopy can be found on the Carl Zeiss Microscopy Online Campus.
